Antibiogram, Genomic and Phylogeny of Stool and Seafood Isolates from Some Cholera-Prone Coastal Communities in Rivers State, Nigeria

Cholera is a significant public health threat across the globe, especially in coastal regions with poor water supply. This study was carried out to determine antibiogram, genomic, and phylogeny of stool seafood isolates from some cholera-prone communities Rivers State, Nigeria. A total 400 samples 42 different were aseptically collected examined using standard microbiology molecular techniques. An antibiogram assayed. Genes for virulence, resistance, relatedness bacteria identified also determined. The susceptibility selected antibiotics. findings showed prevalence rate cholera as follows: 16% Kaa, 30% Andoni, 4% Ogu/Bolo, 10% Abua/Odual. susceptible Ciprofloxacin Gentamycin 94.12% each while 100% resistance recorded against Amoxicillin-clavulanic acid, Amikacin 88.24% Colistin. For sea foods, gentamycin ciprofloxacin 91.43% 82.86% respectively. Resistance Colistin (88.57%) Azithromycin (82.86%). Testing presence 16SrRNA genes that all positive 1500 bp gene band size. TEM, OXA, SHV, CTX-M resistant detected whereas virulence TDH AcrB. phylogenetic analysis revealed be Aeromonas dhakensis, Vibrio parahaemolyticus, azureus, Providencia rettgeri, they Enterobacter sichuanensis, hormaechei, sneebia, Proteus vulgaris. stuartii, mirabilis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae common both samples. has shown not reported cases are caused by cholerae. Therefore, attention should paid other water-borne every outbreak, communities.